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Description
Complement component C5a is a multifunctional proinflammatory mediator produced by complement cascade activation and causes inflammatory responses and cytokine production in septic shock, allergy, trauma, ischemic heart disease, pain, neurodegenerative diseases, and several other autoimmune diseases. C5a is rapidly cleaved to the C5a-DesArg form by the endogenous serum carboxypeptidase enzyme.
The C5a ELISA Kit can be used for in vitro determination of mouse C5a in different tissue samples including skin, muscle, nervous system and other biological samples. The microplate is coated with murine C5a monoclonal antibody. C5a present in the sample or standard binds to the antibodies absorbed to the microwell. A biotin-conjugated anti-murine C5a antibody is then added and binds to murine C5a captured by the first antibody. After adding streptavidin-horseradish peroxidase (HRP) an antibody-antigen-antibody “sandwich” is produced. Finally, substrate solution reactive with HRP is added to the well, exhibiting a blue color in direct proportion to the amount of C5a in the initial sample. The reaction is terminated by the addition of an acid, and absorbance is measured at 450nm. The concentration of C5a in the samples is determined by comparing the O.D. of the samples to the standard curve.
Applications