Tribioscience Leucine dehydrogenase, Enzyme Activity (TBP0033)

Tribioscience Leucine dehydrogenase, Enzyme Activity (TBP0033)

Research Use Only. Not intended for human use.

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Preparation and Specification

Appearance: White amorphous powder, lyophilized

Activity: GradeⅡ 20U/mg-solid or more (containing approx. 70% of stabilizers)

Contaminants: Leucylpeptide decomposing enzymes

(Leu-Val) ≤1.0×10⁻²%

(Leu-Gly-Gly) ≤1.0×10⁻²%

NADH oxidase ≤1.0×10⁻²%

Stabilizers: 2-Mercaptoethanol, L-cysteine, dithiothreitol, ethylenediaminetetraacetate

Properties

Stability: Stable at -20°C for at least One year

Molecular weight: 245,000

Michaelis constants: 1.0×10⁻³M (L-Leucine), 3.9×10⁻⁴M (NAD⁺), 3.5×10⁻⁵M (NADH),

3.1×10⁻⁴M [α-Ketoisocaproate (α-KIC)], 2.0×10⁻¹M (NH₃)

Structure: 6 subunits per enzyme molecule

Inhibitors: Na₂S, Hg⁺⁺, Cu⁺⁺, Co⁺⁺, Mg⁺⁺, p-chloromercuribenzoate

Optimum pH: 10.5-10.8 (L-Leu→α-KIC), 9.4 (α-KIC→L-Leu)

Optimum temperature: above 70°C

pH Stability: pH 5.5-10.5 (25°C, 20hr)

Thermal stability: below 60°C (pH 6.9, 10min)

Applications