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Lactate dehydrogenase (LDH) is a cytosolic enzyme present in nearly all living cells. It is an oxidoreductase catalyzing the interconversion of lactate and pyruvate. LDH is released upon damage of the cytoplasmic membrane. The measurement of LDH release is a well-accepted assay to estimate tissue or cell damage and cell cytotoxicity.
The Tribo™ LDH Colorimetric Assay Kit uses WST-8, which is oxidized to generate formazan to measure the activity of LDH released from damaged cells. The generated signal at OD 460 nm is proportional to the LDH released from the lysed cells. The kit provides the easiest and most accurate approach to measure LDH activity.
Fig. 1: LDH Assay Principle
Applications
- Quantify lactate dehydrogenase in a variety of samples such as serum, plasma tissues, cells, and cell culture medium.
- This kit is designed in a format for a variety of sample types. To measure LDH released into a cell culture medium in a cytotoxicity experiment, we recommend TBS2002, LDH Cytotoxicity Colorimetric Assay.
Storage conditions
Store the Reagent at –20°C protected from light. Shelf life: 12 months.
For Research Use only
Fig. 2: LDH Standard Curve
