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L-Lactate is the major stereo-isomer of lactate formed in human intermediary metabolism and is present in blood. Depending on the level of exercise, blood levels of lactate can vary between 1 and 20 mM. Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate level is a good indicator of the balance between tissue oxygen demand and utilization. L-Lactate accumulates in lactic acidosis which is associated with poor prognosis and increased mortality in patients with critical illness, sepsis and liver failure.
Tribioscience’s Lactate Fluorometric Assay Kit is a simple, sensitive, and fast assay that measures the total amount of L-Lactate in biological samples. Lactate is oxidized by lactate oxidase to pyruvate and hydrogen peroxide. Horseradish peroxidase (HRP) catalyzes the reaction between hydrogen peroxide and the specific probe to yield a final product measured by the fluorometric method at Ex/Em=530/590 nm.
Applications
- Measure L-lactate in a variety of samples.
Storage Conditions
Store the Reagent at -20°C, protect it from light. Shelf life: 12 months.
For Research Use only
Standard Curve
