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The Fast Human MIP-1α ELISA is a solid phase ELISA designed to measure human MIP-1α levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The measurement can be finished in 1 hour, not needing 4-5 hours (Fig. 1). The detection range is from 7 to 500 pg/mL. The levels of human MIP-1α samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human MIP-1α protein.
Typical Data
This standard curve(R2=0.9985) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MOD) of human MIP-1α is typically 5 pg/ml.
The Intra-assay CV is 4.9%, the Inter-assay CV is 8.1%.
Specificity
This assay recognizes natural and recombinant human MIP-1α.
For Research Use only
Simple Procedures
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
| Part | Part# | Description | Storage of Opened/Reconstituted |
|---|---|---|---|
| Human MIP-1α Microplate | TBS3234A | 96 well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for human MIP-1α. | The unused wells can be stored the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C. |
| Human MIP-1α Standard | TBS3234B | 60 ul of Recombinant human MIP-1α protein (10ng/mL). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles. |
| Detection A | TBS3234C | 2.1 ml of human MIP-1α antibody. | May be stored for up to 3 months at 2-8 °C. |
| Detection B | TBS3234D | 12 ml of Streptavidin-HRP | |
| Assay Diluent | TBS3234E | 12 ml of a buffered protein base with preservatives. | |
| Wash Buffer | TBS3000W | 12 mL of concentrated solution (10x). | |
| TMB Substrate | TBS3000T | 12 mL of ultra-sensitive TMB substrate. | |
| Stop Solution | TBS3000S | 6 mL of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.