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The Fast Human IL-17A ELISA is a solid phase ELISA designed to measure human IL-17A levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1 hour, not 5-6 hours (Fig. 1). The detection range is from 8 to 2000pg/mL. The levels of human IL-17A samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human IL-17A protein.
Typical Data
This standard curve(R2=0.998) is provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Sensitivity
The minimum detectable dose (MOD) of human IL-17A is typically 6.5 pg/ml. The Intra-CV is 5.39%, and the Inter-CV is < 12%.
Specificity
This assay recognizes natural and recombinant human IL-17A.
No-cross reactivity: Human INF-γ, IL-10, IL-12, IL-16, IL-17B, IL-17C, IL-17D, IL-17F; Mouse IL-17.
For Research Use only
Simple Procedures (Fig. 1)
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
| Part | Part# | Description | Storage of Opened/Reconstituted |
|---|---|---|---|
| Human IL-17A Microplate |
TBS3228A | 96-well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for human IL-17A. |
The unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C. |
| Human IL-17A Standard |
TBS3228B | 60 μL of Recombinant human L-17A protein (100ng/mL). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles. |
| Detection A | TBS3228C | 2.2 mL of human IL-17A antibody. | May be stored for up to 3 months at 2-8 °C. |
| Detection B | TBS3228D | 120 μL of Streptavidin-HRP. | |
| Assay Diluent | TBS3228E | 12 mL of a buffered protein base with preservatives. | |
| 10x Wash Buffer | TBS3000W | 12 mL of concentrated solution (10x). | |
| TMB Substrate | TBS3000T | 12 mL of ultra-sensitive TMB substrate. | |
| Stop Solution | TBS3000S | 6 mL of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use an expired kit for assay.
The kit contains sufficient materials to run an ELISA on one 96-well plate.