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The TriboTM Human C-peptide ELISA is designed to quantitatively detect Human C-peptide levels in different tissues including skin, muscle, neural, serum, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The measurement can be finished in 3 hours, with no need for 4-5 hours (Fig. 1). The detection range is from 2 to 185 pg/mL. The levels of human C-peptide samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human C-peptide protein.
Typical Data
This standard curve(R2=0.9995) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MOD) of human C-peptide is typically 10 pg/ml.
The Intra-assay CV is 3.79% the Inter-assay CV is ˂10%.
Specificity
This assay recognizes natural and recombinant human C-peptide.
For Research Use only
Simple Procedures
Simple Standard Preparation
Standard Curve
Kit Content and Storage Conditions
| Part | Part# | Description | Storage of Opened/Reconstituted |
|---|---|---|---|
| Human C-peptide Microplate | TBS3248A | 96 well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for human C-peptide. | Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C. |
| Human C-peptide Standard | TBS3248B | 15 µl of Recombinant human C-peptide protein (18.5ng/mL). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles. |
| Detection A | TBS3248C | 2.1 ml of Biotin-Human C-peptide antibody. | May be stored for up to 3 months at 2-8 °C. |
| Detection B | TBS3248D | 300 µl of Streptavidin-HRP. | |
| Assay Diluent | TBS3248E | 25 mL of a buffered protein base with preservatives. | |
| Wash Buffer | TBS3000W | 12 mL of concentrated solution (10x). | |
| TMB Substrate | TBS3000T | 12 mL of ultra-sensitive TMB substrate. | |
| Stop Solution | TBS3000S | 6 mL of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.