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10-Acetyl-3,7-dihydroxyphenoxazine (ADHP), also called Amplex Red or Ampliflu Red, is a sensitive and stable fluorogenic substrate for horseradish peroxidase (HRP). In the presence of HRP and H2O2, ADHP generates highly fluorescent resorufin in red color that has a maximum absorption of 570 nm and maximum emission of 585 nm. So far ADHP has been known as the most sensitive and stable fluorogenic probe for detecting HRP and H2O2. It has been widely used to detect peroxidase Activity.
Tribioscience’s Peroxidase Activity Fluorometric / Colorimetric Assay provides a quick, efficient, and sensitive method of peroxidase activity.
Applications:
- Detection of HRP activity on the liquid system.
Key Features:
- Sensitive and accurate: 10 times more sensitive than TMB substrate for HRP detection.
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Simple: Just add- incubate-read style.
Kit Contents:
| Component | 100x Rxns | 200x Rxns |
|---|---|---|
| ADHP Solution | 60 μL | 120 μL |
| Developer | 600 μL | 1200 μL |
| HRP standard (1 U/mL) | 50 μL | 100 μL |
| 1X Assay buffer | 10 mL | 20 mL |
Storage Conditions: Store the kit at -20℃ protected from light.
Shelf Life: 6 months.
For Research Use only
Diagram for HRP Standard Preparation
Fluorometric Example Standard Curve:
Colorimetric Example Standard Curve:
