This enzyme is useful for enzymatic determination of glycerol and triglyceride when coupled with glycerol-3-phosphate oxidase (=G-3-P oxidase) or pyruvate kinase and lactate dehydrogenase, lipoprotein lipase in clinical analysis.
Tribioscience Glycerol kinase, Enzyme Activity (TBP0026)
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Tribioscience Glycerol kinase, Enzyme Activity (TBP0026)
Research Use Only. Not intended for human use.
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Preparation and Specification
Appearance: White amorphous powder, lyophilized
Activity: GradeⅢ 30 U/mg-solid or more
Contaminants: Catalase ≤1.0×10⁻¹%
NADH oxidase ≤1.0×10⁻³%
Adenosine triphosphatase ≦1.0×10⁻³%
Properties
Stability: Stable at -20°C for at least One year
Molecular weight: approx. 220,000 (by gel filtration)
Structure: Four subunits of approx. 58,000
Isoelectric point: 4.3
Michaelis constants: 9.4×10⁻⁵M (Glycerol), 1.3×10⁻⁵M (ATP), 2.1×10⁻³M (Dihydroxyacetone)
Inhibitors: p-Chloromercuribenzoate, Hg⁺⁺, Ag⁺
Optimum pH: 10.0
Optimum temperature: 70°
pH Stability: pH 5.5-10.0 (25°C, 20hr)
Thermal stability: below 65°C (pH 7.5, 30min)
Applications