Tribioscience Glucose dehydrogenase(NAD(P)-dependent), Enzyme Activity (TBP0020)

Tribioscience Glucose dehydrogenase(NAD(P)-dependent), Enzyme Activity (TBP0020)

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Preparation and Specification

Appearance: White amorphous powder, lyophilized

Activity: GradeⅢ 250U/mg-solid or more

Contaminants: NADH oxidase ≤1.0×10⁻³%, α-Glucosidase ≤1.0×10⁻³% Glucose-6-phosphate dehydrogenase ≤1.0×10⁻³%

Properties

Stability: Stable at -20°C for at least One year

Molecular weight: approx. 101,000 (Gel filtration)

Isoelectric point: 4.5

Michaelis constants: NAD⁺linked: 1.38×10⁻²M (D-Glucose) 3.09×10⁻⁴M (NAD⁺)

NADP⁺linked: 1.25×10⁻²M (D-Glucose) 4.07×10⁻⁵M (NADP⁺)

Inhibitors: Ag⁺, Hg²⁺, Monoiodoacetate

Optimum pH: 9.0

Optimum temperature: 55℃

pH Stability: pH 6.0-7.5 (20℃, 16hr)

Thermal stability:  45℃ (15min-treatment with 50mM K-phosphate buffer, pH 7.0)

Applications

This enzyme is useful for enzymatic determination of D-Glucose