Tribioscience Glucose-6-phosphate dehydrogenase, Enzyme Activity (TBP0017)

Tribioscience Glucose-6-phosphate dehydrogenase, Enzyme Activity (TBP0017)

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Preparation and Specification

Appearance: White amorphous powder, lyophilized

Activity: GradeⅢ 200U/mg-solid or more

Contaminants: Creatine phosphokinase ≤1×10⁻³%

Phosphoglucomutase ≤1×10⁻³%

6-Phosphogluconate dehydrogenase ≤5×10⁻³%

Phosphoglucose isomerase ≤1×10⁻²%

Glutathione reductase ≤1×10⁻³%

Hexokinase ≤1×10⁻²%

Myokinase ≤1×10⁻²%

NADH oxidase ≤1×10⁻²%

NADPH oxidase ≤1×10⁻²%

Properties

Stability: Stable at -20°C for at least One year

Molecular weight: approx. 140,000 (by gel filtration)

Michaelis constants: NAD⁺ linked 2.4×10⁻⁴M (NAD⁺), 4.7×10⁻⁴M (G-6-P)

NADP⁺ linked 7.4×10⁻⁶M (NADP⁺), 3.2×10⁻⁴M (G-6-P)

Inhibitors: Metal ions, iodoacetamimide, SDS etc.

Optimum pH: 7.8

Optimum temperature: 50°C-55°C

pH Stability: pH 5.0-11.0 (25°C, 22hr)

Thermal stability: below 50°C (pH 7.8, 30min)

Applications

The enzyme is useful for enzymatic determination of NAD⁺(NADP⁺) and G-6-P, and activities of phosphoglucose isomerase, phosphoglucomutase and hexokinase. The enzyme is also used for enzymatic determination of glucose and creatine phosphokinase activity when coupled with hexokinase.