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Hydrogen Peroxide (H2O2) is a reactive oxygen metabolic byproduct that serves as a key regulator for a number of oxidative stress-related states. It is involved in many pathological processes of diseases such as asthma, inflammatory arthritis, atherosclerosis, diabetic vasculopathy, osteoporosis, neuro-degenerative diseases, Down’s syndrome, and immune system diseases.
Tribioscience’s ADHP-red Hydrogen Peroxide Assay Kit is a sensitive, simple, direct, and HTS-ready colorimetric/fluorometric assay designed to measure H2O2 levels in biological samples. In the presence of Horse Radish Peroxidase (HRP), the ADHP-red probe reacts with H2O2 to produce a resorufin that appears red in color. The resorufin can be measured with the absorption of 570nm (λmax = 570nm) for colorimetric or a highly stable fluorometric assay at maximum emission of 585nm (Ex/Em = 535/587 nm).
Applications:
- Detection of HRP activity on liquid systems such as ELISA.
- Western Blot or other immunoassays.
- Custom packaging and bulk purchase information are available upon request.
Key Features:
- Flexible: suitable for colorimetric and fluorometric methods.
- Accurate: Use 50 µL samples. Detection ranges 0.4-200 µM in 96-well plate for colorimetric assay and fluorometric assay.
- Simple and high-throughput: One-step procedure – Just load-incubate-read. The kit can be used for a robust method.
- Time-saving less than 30 minutes.
Kit Contents:
| Component | 100x Rxns | 200x Rxns |
|---|---|---|
| ADHP Solution | 60μL | 120μL |
| H2O2 Standard (0.88M) | 50μL | 100μL |
| HRP Reagent | 100μL | 200μL |
| 1x Reaction buffer | 10mL | 20mL |
Storage Conditions: Store the kit at -20℃ protected from light.
Shelf Life: 6 months.
For Research Use only
Diagram for H2O2 Standard Preparation:
Fluorometric Example Standard Curve:
Colorimetric Example Standard Curve:
