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Easy Cloning Kit is designed for quick and effective PCR cloning. With our proprietary recombinase enzyme, this kit is especially powerful in directly cloning any single or multiple PCR products into designated vectors effectively without the tedious and limiting tasks of selecting proper restriction enzymes, phosphatases, or ligases.
Applications:
PCR Cloning up to 100 kb
Gene transfer from one vector to another
High-throughput (HTP) PCR cloning
In vitro joining of DNA fragments
Gene insertion, knockout, mutagenesis et. al
Key Features:
- Fast: One step cloning procedure completed within 30 minutes.
- Easy: PCR DNA and linearized vectors immediately used in cloning. No restriction enzymes, phosphatases, or ligases are needed.
- Effective: Long PCR DNA up to 100kb can be efficiently cloned with a success rate >95%
KIT CONTENTS for 10 or 20 Reactions
| Kit Components | TBS4050-10 | TBS4050-20 |
|---|---|---|
| EasyCLoning Enzyme (5 U/μL) | 10μL | 20μL |
| 10x CloneEZ® Buffer | 20μL | 40μL |
| Manual | 1 | 1 |
Storage Conditions:
The kit should be stored at -20°C. It will remain stable for at least one year.