Tribioscience Diaphorase, Enzyme Activity (TBP0015)

Tribioscience Diaphorase, Enzyme Activity (TBP0015)

Buy Tribioscience products from MSE Supplies at the best value. 

Preparation and Specification

Appearance: Yellowish amorphous powder, lyophilized

Activity: Grade III 500 U/mg-solid or more

Contaminants: Myokinase ≤5.0×10⁻¹%, NADH oxidasee ≤1.0×10⁻¹%

Properties

Stability: Stable at -20°C

Molecular weight: approx. 48,000

Michaelis constant: 2.2 × 10-⁴M (NADH), 2.9 × 10-²M(NADPH)

Inhibitors: Fe³⁺, Mn²⁺, Cu²⁺, Pb²⁺

Isoelectric point: 5.0

Optimum pH: 8.0

Optimum temperature: 60°C

pH Stability: 5.0–10.0 (25°C, 20hr)

Thermal stability: below 70°C (pH7.5, 15min)

Substrate specificity: Either NADH or NADPH can be used as a reductant.

Applications

This enzyme is useful for colorimetric determination of NAD(P)H and many dehydrogenases when coupled with various dyes which act as hydrogen acceptors from NAD(P)H.

esterase in clinical analysis.