Tribo™ E. coli DH10 Competent Cells is an E. coli strain DH10B that provides high transformation efficiencies and allows stable replication of high-copy number plasmids. The competent cells can be used in a wide variety of applications for cloning and subcloning. The lacZΔM15 marker provides α-complementation of the β-galactosidase gene, allowing blue/white screening of colonies on plates containing X-gal.
Application
- General cloning and subcloning.
- Blue-white selection.
- Plasmid DNA amplification.
- Plasmid isolation.
- Long-term storage for plasmid stock.
Transformation Efficiency (cfu/Ug)
>1×10^9
Blue-White Screening
Yes
Reduces Recombination
Yes
Improves Plasmid Quality
Yes
recA Deficient
Yes
Strain
K12
Genotype
F–mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK λ–rpsL(StrR) endA1 nupG
Storage
Store the competent cells at -80°C. Shelf life: 6 months.
