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CV-HLVD-LCV Multiplex RT-PCR Detection Kit is used for the detection of cryptic virus (CV), hop latent viroid(HLVD), and lettuce chlorosis (LCV) from the cannabis plant in one step RT-PCR reaction. It combines both reverse transcription (RT) and Taqman probe-based PCR amplification to occur in a single reaction tube. The kit is optimized for the two reactions in a real-time “single step”. This One-step qRT-PCR assay offers the end-users an efficient, easy-to-use, and reliable alternative to conventional “two-step” sequential qRT-PCR for detecting CV, HLVd, and LCV from plant samples.
Tribioscience Multiplex RT-PCR Detection Kit contains all components for RT-PCR reaction including RT-PCR enzymes, primer probes, internal control, positive control, negative control, and buffer. The 3 target genes are labeled with a specific fluorescence color: Cy5 for Cryptic virus, Fam for HLVD, ROX for lettuce chlorosis virus, and Hex for internal control.
Applications
- This kit is used for the detection of CV, HLVd, and LCV infection in plant samples.
Key Features
- One-step complete qRT-PCR in a single tube.
- Reduce contamination in the operating process.
- Accurate detection and quantification of HLVd target gene.
- 4x RT-PCR mix makes it easier to adjust the sample size in a one-tube reaction.
Storage Conditions
Store all components at -20°C in a non-frost-free freezer.
Shelf life is 12 months after receipt. The kit is shipped on ice.