Tribioscience Creatine amidinohydrolase, Enzyme Activity (TBP0014)

Tribioscience Creatine amidinohydrolase, Enzyme Activity (TBP0014)

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Preparation and Specification

Appearance: (-221) White amorphous powder, lyophilized (-229) Clear solution

Activity: GradeⅡ (-221) 4.0U/mg-solid or more (-229) 2000U/ml or more

Contaminants: NADH oxidase ≤5.0×10⁻²%; Catalase ≤2.0%

Stabilizers: Sugars, EDTA

Properties

Stability: (-221) Stable at -20°C for at least One year (-229) Stable at 4°C

Molecular weight: approx. 67,000 (by gel filtration)

Structure: 2 subunits per enzyme molecule

Isoelectric point: 4.5±0.1

Michaelis constant: 4.5×10⁻³ M (Creatine)

Inhibitors: Hg⁺⁺, Cu⁺⁺, Ag⁺, SH reagent (NEM), PCMB

Optimum pH: 6.5-7.5

Optimum temperature: 40-50℃

pH Stability: pH 4.0-10.0 (25℃, 20hr)

Thermal stability: below 50℃ (pH 7.5, 30min)

Applications

This enzyme is useful for enzymatic determination of creatine and creatinine when coupled with creatinine amidohydrolase and sarcosine oxidase in clinical analysis.

esterase in clinical analysis.