This enzyme is useful for enzymatic determination of phospholipids when coupled with phospholipase D and for choline esterase-activity in clinical analysis.
Tribioscience Choline Oxidase, Enzyme Activity (TBP0009)
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Tribioscience Choline Oxidase, Enzyme Activity (TBP0009)
Research Use Only. Not intended for human use.
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Preparation and Specification
Appearance:Â Yellowish amorphous powder, lyophilized
Activity: GradeⅢ　10U/mg-solid or more (containing approx. 20% of stabilizers)
Contaminant: Catalase ≤1.0×102%
Stabilizers:Â EDTA, BSA, amino acids (glycine, sodium glutamate, etc.)
Properties
Stability: Stable at −20℃ for at least one year
Molecular weight:Â approx. 95,000
Isoelectric point: 4.1±0.1
Michaelis constants: 2.84×10-3M (Choline), 5.33×10-3M (Betaine aldehyde)
Structure:Â One mol of FAD is covalently bound to mol of the enzyme
Inhibitors: p-Chloromercuribenzoate, Cu＋＋, Co＋＋, Hg＋＋, Ag＋
Optimum pH: 8.0−8.5
Optimum temperature: 40−45℃
pH Stability: pH 7.0−9.0 (30℃, 2 hr)
Thermal stability: below 37℃ (pH 7.5, 10min)
Applications