Tribioscience Cholesterol oxidase, Enzyme Activity (TBP0013)

Tribioscience Cholesterol oxidase, Enzyme Activity (TBP0013)

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Preparation and Specification

Appearance: Yellowish amorphous powder, lyophilized

Activity: GradeⅢ　12U/mg-solid or more

Contaminants: Catalase ≤1.0×10^-1%, Cholesterol esterase ≤1.0×10^-2%

Stabilizers: BSA, amino acids

Properties

Stability: Stable at −20℃ for at least one year

Molecular weight: approx. 55,000 (by gel-filtration)

Isoelectric point: 4.6±0.1, 4.9±0.1 and 5.2±0.1

Michaelis constant: 2.1×10^-5M (Cholesterol)

Inhibitors: Ionic detergents, Ag^＋, Hg^＋＋

Optimum pH: 7.0−8.0

Optimum temperature: 60℃

pH Stability: pH 5.0−10.0 (25℃, 20hr)

Thermal stability: below 55℃ (pH 7.0, 15min)

Applications

This enzyme is useful for enzymatic determination of cholesterol in serum when coupled with cholesterol

esterase in clinical analysis.