Product Details
Formal Name: (3aS,4S,6aR)-Hexahydro-N-[2-(4-hydroxyphenyl)ethyl]-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide
Alternate Names: Biotin-phenol
Molecular Formula: C18H25N3O3S
Formula Weight: 363.47
CAS Number: 41994-02-9
Purity: >98%
Formulation: powder
Solubility: Soluble in DMSO (up to 35 mg/ml) or in Ethanol (up to 20 mg/ml)
Storage: -20°C
Stability: ≥ 1 year.
Applications
Signal amplification reagent for antibody assays
Functions
Reagent for use in catalyzed reporter deposition (CARD) signal amplification protocols in a variety of immunoassays in which, horseradish peroxidase- catalyzed deposition of biotinyl tyramide is detected with labeled streptavidin. Widely used for signal amplification in fluorescent in situ hybridization (FISH) protocols. May be used for signal amplification in a sandwich ELISA for quantification of α-tubulin isotypes. Reagent for “self-biotinylation” of DNA G-quadruplexes. May be used with APEX-mediated biotin labeling to identify protein-protein interactions.
Application Procedures
First dissolved in DMSO (up to 35 mg/ml) or in Ethanol (up to 20 mg/ml), then diluted to aqueous buffer. Solutions in
DMSO or ethanol may be stored at -20°C for up to 1 month.