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Description: Human-Rabbit chimeric antibody is made by fusion of the antigen binding region to rabbit anti-His Tag monoclonal antibody, with the constant domain of human IgG1. The antibody is recognized only by an anti-Human IgG secondary antibody.
Specificity: This antibody reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with any endogenous protein in mammalian or bacteria cells.
Host Animal: Rabbit.
Isotype: IgG.
Immunogen: Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.
Format: Purified, liquid.
Purification: Protein an affinity purified from an animal origin–free culture supernatant.
Concentration: 1.0 mg/ml.
Unit Size: 100 µg
Buffer: 50% Glycerol/PBS with 0.09% sodium azide.
Application:
For ELISA: Capturer: 0. 1 µg/mL – 2 µg/mL; Detector: 0.01 µg/mL – 0.5 µg/mL.
For WB: 0.1 µg/mL – 1 µg/mL.
Storage: Store at -20℃ for 1 year.