SMCC Activated Peroxidase (HRP)
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SMCC Activated Peroxidase (HRP)
Technical Information
| Description | Maleimide Activated Horseradish Peroxidase (HRP) is commonly used to facilitate the conjugation of HRP to proteins, peptides and ligands that contain sulfhydryl (-SH) groups for use as an enzyme-linked detection reagent. This product consists of HRP that has been modified with the hetero bi-functional cross linker Sulfo-SMCC and it generally contains several maleimide groups per HRP molecule while maintaining most of the HRP activity. After conjugation, the activated HRP will form a covalent interaction with the peptides or ligands containing sulfhydryl groups and can be used as a HRP probe for detection in different assays such as ELISA and western blot etc. |
| Molecular Weight | 40 kDa |
| Activation Method | Sulfo-SMCC |
| Binding capacity | 2-4 mg of peptide (average MW of 1000-3000) per 2 mg HRP |
| Reconstitute | Reconstitute in distilled water at the concentration of 2-5 mg/mL with gentle stirring. |
Shipping & Storage Information
| Storage | -20°C, shading light |
| Shipping | Blue ice |
| Expiration date | 6 months |