Recombinant Rat LAG3/CD223 Protein (His Tag)
SKU: PKSR030201-100
Recombinant Rat LAG3/CD223 Protein (His Tag)
| SKU # | PKSR030201 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | LAG3 |
| Species | Rat |
| Expression Host | HEK293 Cells |
| Sequence | Met1-Leu442 |
| Accession | Q5BK54 |
| Calculated Molecular Weight | 47.1 kDa |
| Observed Molecular Weight | 55 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile 20 mM NaAc, 500 mM NaCl, 10 % Glycerol, 0.02 % tween 20, pH 5.5 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
LAG3, also known as CD223 and Lymphocyte activation gene 3, belongs to immunoglobulin (Ig) superfamily. LAG3 contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. It is selectively expressed in activated T and NK cells. LAG3 has a negative regulatory function in T cells. It also acts as as a new marker of T cell induced B cell activation. As a soluble molecule, LAG3 activates antigen-presenting cells through MHC class II signalling, leading to increased antigen-specific T-cell responses in vivo.