Recombinant Mouse YM1/ECF-L/Chil3 Protein (Pro105Ser)(His Tag)
SKU: PKSM041348-50
Recombinant Mouse YM1/ECF-L/Chil3 Protein (Pro105Ser)(His Tag)
| SKU # | PKSM041348 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | Chitinase-like protein, Chil, Chi3l, Chitinase-like protein 3, Chi3l3, Ym1, Chil3 |
| Species | Mouse |
| Expression Host | HEK293 Cells |
| Sequence | Tyr22-Tyr398 |
| Accession | O35744 |
| Calculated Molecular Weight | 43.4 kDa |
| Observed Molecular Weight | 42-50 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 6% Sucrose, 2% Glycine, 0.05% Tween 80, pH7.5. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Chitinase 3-like 3 gene, also known as YM1 and ECF-L, encodes a precursor protein with 398 amino acid residues with a 21 residue signal sequence. Chitinase 3-like 3 protein is a lectin that binds saccharides with a free amino group, such as glucosamine or galactosamine. Binding to oligomeric saccharides is much stronger than binding to mono- or disaccharides. Also binds heparin and G1cN oligomers, and is produced primarily by macrophages during inflammation. It has chemotactic activity for T-lymphocytes, bone marrow cells and eosinophils.