Recombinant Mouse Nogo Receptor/NgR Protein (His Tag)(Active)
SKU: PKSM040861-100
Recombinant Mouse Nogo Receptor/NgR Protein (His Tag)(Active)
| SKU # | PKSM040861 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | NOGOR, NgR, NgR1, Rtn4r |
| Species | Mouse |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Ser 447 |
| Accession | NP_075358.1 |
| Calculated Molecular Weight | 47.0 kDa |
| Observed Molecular Weight | 65 kDa |
| Tag | C-His |
| Bio-activity | 1. Immobilized recombinant Mouse RTN4R at 2 μg/ml (100 μl/well) can bind biotinylated human RTN4 (GST Tag) with a linear range of 0.04-0.625 μg/ml. |
Properties
| Purity | > 97 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Reticulon 4 receptor (RTN4R), also known as Nogo-66 Receptor (NgR), is a glycosylphosphoinositol (GPI)-anchored protein that belongs to the Nogo recptor family including three members. Mouse RTN4R cDNA contains 10 LRP (Leucine-rich) repeats. RTN4R is expressed predominantly in neurons and their axons in the central nervous systems (CNS). As a receptor for myelin-derived proteins Nogo, myelin-associated glycoprotein (MAG), and myelin oligodendrocyte glycoprotein (OMG), RTN4R mediates axonal growth inhibition and may play a role in regulating axonal regeneration and plasticity in the adult CNS. It has been shown that RTN4R performs its inhibitory actions by interacting with the p75 neurotrophin receptor (p75NTR), a TNFRSF member also known for modulating the activities of the Trk family and for inducing apoptosis in neurons and oligodendrocytes. RTN4R may be proposed as a potential drug target for treatment of various neurological conditions such as spinal cord injury, CNS lesions, peripheral nerve injury, stroke and Alzheimer's disease (AD). Additionally, RTN4R may play a role in regulating the function of the gap junctions.