Recombinant Mouse IL1R1/CD121a Protein (His Tag)(Active)
SKU: PKSM040477-100
Recombinant Mouse IL1R1/CD121a Protein (His Tag)(Active)
| SKU # | PKSM040477 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | D2S, Interleukin-1 receptor type, IL1-R, mIL-1R, CD121 antigen-like family member A, CD121a, CD121b, D2S1473, IL-1 RI, IL1R, Il1r-1, IL1-R1, IL-1R-1, IL-1R-alpha, IL-1RT1, IL-1RT-1, IL-iR, Interleukin-1 receptor alpha, Interleukin-1 receptor type 1, mIL-1R1, P80, Il-1r1, Il1ra, Il1r1, IL-1R type I, IL1R, Interleukin 1 receptor, interleukin 1 receptor type 1, mIL-1R1, type I |
| Species | Mouse |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Lys 338 |
| Accession | P13504 |
| Calculated Molecular Weight | 38.7 kDa |
| Observed Molecular Weight | 55-60 kDa |
| Tag | C-His |
| Bio-activity | 1. Measured by its ability to bind mouse IL1B in a functional ELISA. 2. Immobilized mouse IL1A at 10 μg/mL (100 μl/well) can bind mouse IL1R1, The EC50 of mouse IL1R1 is 0.13 μg/mL. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Interleukin 1 receptor, type I (IL-1R1) also known as CD121a (Cluster of Differentiation 121a), is an interleukin receptor. IL-1R1/CD121a is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA). IL-1R1/CD121a is an important mediator involved in many cytokine induced immune and inflammatory responses. This protein has been characterized by pharmacological and molecular techniques in the mouse brain. The spindle-shaped astrocytes enclose the wound, separating the healthy from damaged neural tissue. The shape change and subsequent repair processes are IL-1β activity-dependent, acting through the IL-1 type 1 receptor (IL-1R1), as co-application of the IL-1type 1 receptor antagonist protein (IL-1ra) blocks IL-1β induced effects. In the spleen, a slight increase in IL-1R AcP and IL-1R1 was observed during the first hours following LPS stimulation. In conclusion, IL-1R AcP mRNA is expressed in the brain and in other tissues where IL-1R1/CD121a transcripts are found. However, the regulation of its expression is distinct from IL-1R1/CD121a. The high level of expression and the lack of regulation of IL-1R AcP transcripts in the brain under inflammatory conditions suggest that the protein might be constitutively expressed in excess.