Recombinant Mouse EpCAM/TROP-1 Protein (Fc Tag)(Active)
SKU: PKSM041339-50
Recombinant Mouse EpCAM/TROP-1 Protein (Fc Tag)(Active)
| SKU # | PKSM041339 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CD326, E, EGP, EGP-2, Epithelial cell adhesion molecule, Neurotrophic tyrosine kinase receptor-related 1, ROR1, TROP1, Tumor-associated calcium signal transducer 1, receptor tyrosine kinase-like orphan receptor 1, tyrosine-protein kinase transmembrane receptor ROR1 |
| Species | Mouse |
| Expression Host | HEK293 Cells |
| Sequence | Gln24-Thr266 |
| Accession | Q99JW5 |
| Calculated Molecular Weight | 54.8 kDa |
| Observed Molecular Weight | 60-80 kDa |
| Tag | C-Fc |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Epithelial Cellular Adhesion Molecule (Ep-CAM), also known as EGP314, mEGP314, Protein 289A, Tumor-associated calcium signal transducer 1, CD326, belongs to the EPCAM family. Its’ monomer subunit structure interacts with phosphorylated CLDN7. Ep-CAM may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. It plays a role in embryonic stem cells proliferation and differentiation. It also up-regulates the expression of FABP5, MYC and cyclins A and E. The post-translational modification glycosylation at Asn-198 is crucial for protein stability.