Recombinant Mouse ECM1 Protein (His Tag)(Active)
SKU: PKSM040729-100
Recombinant Mouse ECM1 Protein (His Tag)(Active)
| SKU # | PKSM040729 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | AI663821, p85 |
| Species | Mouse |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Glu 559 |
| Accession | Q61508-1 |
| Calculated Molecular Weight | 62.5 kDa |
| Observed Molecular Weight | 90-95 kDa |
| Tag | C-His |
| Bio-activity | Measured by its ability to bind human MMP-9 in a functional ELISA. |
Properties
| Purity | > 88 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Extracellular matrix protein 1 (ECM1) is a secreted glycoprotein and playing a pivotal role in endochondral bone formation, angiogenesis, and tumour biology. Three splice variants have been identified: ECM1a (540 aa) is most widely expressed, with highest expression in the placenta and heart; ECM1b (415 aa) is differentiation-dependent expressed and found only in tonsil and associated with suprabasal keratinocytes; ECM1c (559 aa) accounts for approximately 15% of skin ECM1. Although ECM1 is not tumor specific, is significantly elevated in many malignant epithelial tumors and is suggested as a possible trigger for angiogenesis, tumor progression and malignancies. It also has been shown to regulate endochondral bone formation, skeletal development and tissue remodeling.