Recombinant Mouse CD3D & CD3E Heterodimer Protein
SKU: PKSM040323-50
Recombinant Mouse CD3D & CD3E Heterodimer Protein
| SKU # | PKSM040323 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | T3d |
| Species | Mouse |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Ala 105&Met 1-Asp 108 |
| Accession | P04235&P22646 |
| Calculated Molecular Weight | 76 kDa |
| Observed Molecular Weight | 45-50 kDa |
| Tag | C-Flag-Fc & C-His-Fc |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
T-cell surface glycoprotein CD3D & CD3E, also known as CD3 delta & CD3 epsilon chain, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.