Recombinant Mouse CD27/TNFRSF7 Protein (Fc & His Tag)
SKU: PKSM041253-50
Recombinant Mouse CD27/TNFRSF7 Protein (Fc & His Tag)
| SKU # | PKSM041253 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CD27 antigen, CD27L receptor, S152, T-cell activation antigen CD27, Tnfrsf7, Tp55, Tumor necrosis factor receptor superfamily member 7 |
| Species | Mouse |
| Expression Host | HEK293 Cells |
| Sequence | Pro24-Arg182 |
| Accession | P41272 |
| Calculated Molecular Weight | 45.8 kDa |
| Observed Molecular Weight | 50-70 kDa |
| Tag | C-Fc-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Mouse CD27 is a single-pass type I membrane protein which is a member of the tumor necrosis factor receptor superfamily. CD27 is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. CD27 transduces signals that lead to the activation of NF-κB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.