Recombinant Mouse CD226/DNAM-1 Protein (His Tag)
SKU: PKSM041233-50
Recombinant Mouse CD226/DNAM-1 Protein (His Tag)
| SKU # | PKSM041233 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | BC051526, CD226, CD226 antigen, CD226 molecule, DNAM-1, DNAM1, DNAM1 adhesion glycoprotein, DNAX accessory molecule 1, DNAX accessory molecule-1, PTA1, Pta1, T lineage-specific activation antigen 1 antigen, TLiSA1, platelet and T cell activation antigen 1 |
| Species | Mouse |
| Expression Host | HEK293 Cells |
| Sequence | Glu19-Pro254 |
| Accession | Q8K4F0 |
| Calculated Molecular Weight | 27.6 kDa |
| Observed Molecular Weight | 35-50 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Mouse DNAX accessory molecule-1(DNAM-1) is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily. As an activating receptor, it interacts with the ligands CD155 and CD112, and activates natural killer (NK) cells via its immu-noreceptor tyrosine-based activatory motif (ITAM). Mature mouse DNAM-1 has extracellular domain (ECD) that contains two Ig-like C2-set domains, and possesses a cytoplasmic region that contains motifs for binding PDZ domains. DNAM-1 is expressed on several lymphoid and myeloid cell types and interacts with CD155/PVR and Nectin-2/CD112. Ligation of DNAM-1 promotes the activation of NK cells, CD8+ T cells, and mast cells, induces dendritic cell maturation, initiates megakaryocyte and activated platelet adhesion to vascular endothelial cells, and stimulates monocyte extravasation. Conversely, it inhibits the formation of osteoclasts. Platelet-endothelium interactions that are mediated by DNAM-1 enable the metastasis of tumor cells to the lung.