Recombinant Human Proenkephalin-B/PDYN Protein (His Tag)
SKU: PKSH033558-50
Recombinant Human Proenkephalin-B/PDYN Protein (His Tag)
| SKU # | PKSH033558 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | ADCA, Big Dyn, Dynorphin B, PDYN, PENKB, SCA23 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Asp21-Ala254 |
| Accession | P01213 |
| Calculated Molecular Weight | 27.3 kDa |
| Observed Molecular Weight | 35 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 4mM HCl. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Proenkephalin-B(PDYN), belongs to the opioid neuropeptide precursor family. The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing. Leu-enkephalins, which is a type of Proenkephalin-B, compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. Dynorphin peptides differentially regulate the kappa opioid receptor. Dynorphin A has a typical opiod activity, it is 700 times more potent than Leu-enkephalin.