Recombinant Human Insulin Protein (Active)
SKU: PKSH032458-100
Recombinant Human Insulin Protein (Active)
| SKU # | PKSH032458 |
| Expression Host | Yeast |
Description
| Synonyms | IDDM, IDDM1, IDDM2, ILPR, INS, IRDN, MODY10 |
| Species | Human |
| Expression Host | Yeast |
| Sequence | Phe25-Lys53&Gly90-Asn110 |
| Accession | P01308 |
| Calculated Molecular Weight | 7.1 kDa |
| Observed Molecular Weight | 6 kDa |
| Tag | None |
| Bio-activity | Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. The ED50 for this effect is typically 0.2-1 µg/mL. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake.