Recombinant Human IL-32 alpha protein(N-His)(active)
SKU: PKSH034116-100
Recombinant Human IL-32 alpha protein(N-His)(active)
| SKU # | PKSH034116 |
| Expression Host | E.coli |
Description
| Synonyms | TAIFa, IL-32alpha, IL-32beta, IL-32delta, IL-32gamma, TAIFb, TAIFc, TAIFd, IL32, NK4, TAIF, IL 32alpha, IL 32beta, IL 32delta, IL 32gamma, IL-32, Interleukin 32, Interleukin 32 small, Interleukin 32 theta, Interleukin-32, interleukin-32 eta, Natural killer cell transcript 4, Natural killer cells protein 4, NK 4, OTTHUMP00000236040, OTTHUMP00000236041, OTTHUMP00000236042, OTTHUMP00000236043, OTTHUMP00000236044, OTTHUMP00000236045, OTTHUMP00000236046, OTTHUMP00000236047, OTTHUMP00000236048, OTTHUMP00000236049, OTTHUMP00000236050, OTTHUMP00000236051, OTTHUMP00000236052, OTTHUMP00000236053, OTTHUMP00000236054, OTTHUMP00000241545, OTTHUMP00000241602, OTTHUMP00000241603, OTTHUMP00000241604, OTTHUMP00000241605, OTTHUMP00000241606, OTTHUMP00000241607, OTTHUMP00000241608, OTTHUMP00000241609, OTTHUMP00000241610, OTTHUMP00000241611, OTTHUMP00000241612, Tumor necrosis factor alpha-inducing factor |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Lys 131 |
| Accession | P24001-4 |
| Calculated Molecular Weight | 15.7 kDa |
| Observed Molecular Weight | 18 kDa |
| Tag | C-His |
| Bio-activity | Measure by its ability to induce TNF alpha secretion in RAW264.7 cells. The ED50 for this effect is <10 μg/mL. |
Properties
| Purity | > 98 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 0.1 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS,pH 8.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
IL-32 is a recently discovered cytokine that induces various proinflammatory cytokines (TNF-alpha, IL-1beta, IL-6) and chemokines in both human and mouse cells through the NF-kappaB and p38 MAPK inflammatory signal pathways. It is regulated robustly by other major proinflammatory cytokines and is crucial to inflammation and immune responses. Four of the IL-32 isoforms (alpha, beta, gamma, and delta) are the most representative IL-32 transcripts, and the gamma isoform of IL-32 is the most active, although all isoforms are biologically active. IL-32, a cytokine produced mainly by T, natural killer, and epithelial cells induces significant amounts of TNFalpha and MIP-2 and increases the production of both cytokines in a dose-dependent manner. IL-32 has been implicated in inflammatory disorders, Mycobacterium tuberculosis infections, inflammatory bowel disease, and influenza A virus infection, as well as in some autoimmune diseases, such as rheumatoid arthritis, ulcerative colitis, and in the human stomach cancer, human lung cancer, and breast cancer tissues. Thus, IL-32 expression might be valuable as a biomarker for cancer.