Recombinant Human Galactolipase/PLRP2 Protein (His Tag)
SKU: PKSH030573-100
Recombinant Human Galactolipase/PLRP2 Protein (His Tag)
| SKU # | PKSH030573 |
| Expression Host | Baculovirus-Insect Cells |
Description
| Synonyms | Galactolipase, PL-RP2, PLRP2, PNLIPRP2, Pancreatic Lipase-Related Protein 2 |
| Species | Human |
| Expression Host | Baculovirus-Insect Cells |
| Sequence | Met 1-Cys469 |
| Accession | P54317 |
| Calculated Molecular Weight | 51.5 kDa |
| Observed Molecular Weight | 57 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile 20mM PB, 500mM NaCl, 10% glycerol, pH7.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Galactolipase; also known as PNLIPRP2; is a lipase with broad substrate specificity. It can hydrolyze both phospholipids and galactolipids. Galactolipase acts preferentially on monoglycerides; phospholipids and galactolipids. It also hydrolyses milk fat with a lower catalytic efficiency. The expressed galactolipase shows a lipolytic activity that is; however; only marginally dependent on the presence of colipase. The lipolytic activity of pancreatic extracts and human pancreatic juice on Labrasol is mainly due to the combined action of carboxyl ester hydrolase and galactolipase.