Recombinant Human CD59 (C-6His)
SKU: PKSH034028-50
Recombinant Human CD59 (C-6His)
| SKU # | PKSH034028 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | 16.3A5, 1F5, 1F5 antigen, 20 kDa homologous restriction factor, CD59, CD59 antigen, CD59 glycoprotein, EL32, FLJ38134, FLJ92039, G344, HRF-20, HRF20, MAC-IP, MACIF, MIC11, MIC11MSK21, MIN1, MIN2, MIN3, MIRL, p18-20 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Leu26-Asn102 |
| Accession | P13987 |
| Calculated Molecular Weight | 9.8 kDa |
| Observed Molecular Weight | 15-20 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
CD59, also known as membrane attack complex inhibition factor (MACIF), Protectin, 1F5 antigen, HRF-20 and MIRL, is an approximately 20 kDa GPI anchored glycoprotein that is an important regulator of the complement system in blood. CD59 is a potent inhibitor of the complement membrane attack complex (MAC) action. CD59 was first identified as a regulator of the terminal pathway of complement. It acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. CD59 is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.