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Recombinant E.coli Beta-Glucuronidase/Uida/Beta-Gus Protein (His Tag)
SKU: PKSQ050059-50
Recombinant E.coli Beta-Glucuronidase/Uida/Beta-Gus Protein (His Tag)
| SKU # | PKSQ050059 |
| Expression Host | E.coli |
Description
| Synonyms | Beta-glucuronidase, GUS, uidA |
| Species | E.coli |
| Expression Host | E.coli |
| Sequence | Met1-Gln603 |
| Accession | P05804 |
| Calculated Molecular Weight | 69.9 kDa |
| Observed Molecular Weight | 72 kDa |
| Tag | N-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of PBS, pH7.4. |
| Reconstitution | Not Applicable |
Background
Beta-D-glucuronidase from E.coli is a highly specific enzyme in hydrolyzing glucuronides. Beta-D-glucuronidase (GLUase) activity can be measured as the rate of production of fluorescent methylumbelliferone (MU), resulting from the hydrolysis of the substrate 4-methylumbelliferyl-β-d-glucuronide (MUGLU), which is an effective and rapid method for detection and verification of E. coli in food, water, and environmental samples. High purity recombinant beta-Glucuronidase is used in research, biochemical enzyme assays and in vitro diagnostic analysis, detecting a wide range of drugs such as opioids, benzodiazepines, steroids, cannabinoids, and others.