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Recombinant Cynomolgus LAG-3/CD223 Protein (His Tag)
SKU: PKSQ050011-50
Recombinant Cynomolgus LAG-3/CD223 Protein (His Tag)
| SKU # | PKSQ050011 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CD223, LAG-3, LAG3, Lymphocyte Activating 3 |
| Species | Cynomolgus macaques |
| Expression Host | HEK293 Cells |
| Sequence | Pro23-Leu450(Pro74) |
| Accession | XP_005570011.1 |
| Calculated Molecular Weight | 47.3 kDa |
| Observed Molecular Weight | 55-65&140 kDa |
| Tag | N-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Lymphocyte Activating 3(LAG3) belongs to immunoglobulin (Ig) superfamily, Macaca fascicularis LAG3 cDNA encodes 533 amino acids (aa) that include a 17 aa signal sequence, a 451 aa extracellular domain (ECD) with two Ig-like domains, a transmembrane region and a highly charged cytoplasmic region. LAG3's main ligand is MHC class II, to which it binds with higher affinity than CD4. The protein negatively regulates cellular proliferation, activation, and homeostasis of T cells, in a similar fashion to CTLA-4 and PD-1 and has been reported to play a role in Treg suppressive function.