RBFOX3 Polyclonal Antibody Store at -20°C
SKU: E-AB-12595-200
RBFOX3 Polyclonal Antibody Store at -20°C
| SKU # | E-AB-12595 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Applications | WB, IHC |
Product Details
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide of human RBFOX3 |
| Abbre | RBFOX3 |
| Synonyms | FLJ56884, FLJ58356, FOX3NeuN, Fox-1 homolog C, Fox3, HRNBP3, NEUN, RFOX3, RNA binding protein, RNA binding protein fox-1 homolog 3, Rbfox3, fox 1 homolog (C. elegans) 3, fox1 homolog C, hexaribonucleotide binding protein 3, neuronal nuclei |
| Swissprot | |
| Calculated MW | 34 kDa |
| Observed MW | Refer to figures |
| Cellular Localization | Nucleus. Cytoplasm. |
| Concentration | 0.1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Epigenetics and Nuclear Signaling, Neuroscience, Tags and Cell Markers |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
Related Reagents
| Applications | Recommended Dilution |
| WB | 1:200-1:1000 |
| IHC | 1:50-200 |
Background
Immunoprecipitation and mass spectrometry of the two major NeuN species at 45–50 kDa identified both as the RNA binding protein Rbfox3 (a member of the Fox family of alternative splicing factors), confirming and extending the identification of the 45 kDa band as Rbfox3. Mapping of the anti-NeuN reactive epitopes in both R3hdm2 and Rbfox3 reveals a common proline- and glutamine-rich domain that lies at the N-terminus of the Rbfox3 protein. Nuclear Rbfox3 isoforms can also enhance the inclusion of cryptic exons in the Rbfox2 mRNA, resulting in nonsense-mediated decay of the message, thereby contributing to the negative regulation of Rbfox2 by Rbfox3 through a novel mechanism.