Phospho-CHEK2 (Thr68) Polyclonal Antibody
SKU: E-AB-20843-200
Phospho-CHEK2 (Thr68) Polyclonal Antibody
| SKU # | E-AB-20843 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Applications | WB, IHC-p, IF |
Product Details
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC-p, IF |
| Clonality | Polyclonal |
| Immunogen | Synthesized peptide derived from human Chk2 around the phosphorylation site of Thr68 |
| Synonyms | CDS 1, CHEK 2, CHK2, CHK2 checkpoint homolog, CHK2 checkpoint homolog (S. pombe), Cds1, Cds1 homolog, Checkpoint kinase 2, Checkpoint like protein CHK2, Chek2, Chk 2, HuCds 1, LFS 2, LFS2, PP1425, RAD 53, RAD53, Rad53 homolog, Ser, Serine/threonine protein kinase Chk2, hCds1 |
| Swissprot | |
| Calculated MW | 61 kDa |
| Observed MW | 61 kDa |
| Cellular Localization | Nucleus, Nucleus, Isoform 10 is present throughout the cell and Nucleus>PML body, Nucleus>nucleoplasm, Recruited into PML bodies together with TP53. |
| Tissue Specificity | High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Epigenetics and Nuclear Signaling |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
Related Reagents
| Applications | Recommended Dilution |
| WB | 1:500-1:2000 |
| IHC | 1:100-1:300 |
| IF | 1:50-1:200 |
Background
Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.