Phospho-Catenin Beta (Ser37) Polyclonal Antibody
SKU: E-AB-20828-200
Phospho-Catenin Beta (Ser37) Polyclonal Antibody
| SKU # | E-AB-20828 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Applications | WB, IHC-p, IF |
Product Details
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC-p, IF |
| Clonality | Polyclonal |
| Immunogen | Synthesized peptide derived from human Catenin-β around the phosphorylation site of Ser37 |
| Synonyms | 88kDa, Beta catenin, Beta-catenin, CATNB, CHBCAT, CTNB1, CTNNB, CTNNB1, Cadherin associated protein, Catenin (cadherin associated protein), Catenin beta 1, Catenin beta-1, DKFZp686D02253, FLJ25606, FLJ37923, OTTHUMP00000162082, OTTHUMP0000016522, OTTHUMP00000165222, beta 1 |
| Swissprot | |
| Calculated MW | 85 kDa |
| Observed MW | 85 kDa |
| Cellular Localization | Cytoplasm. Nucleus. Cytoplasm>cytoskeleton. Cell junction>adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization. |
| Tissue Specificity | Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Cardiovascular, Neuroscience, Signal Transduction, Stem Cells |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
Related Reagents
| Applications | Recommended Dilution |
| WB | 1:500-1:2000 |
| IHC | 1:100-1:300 |
| IF | 1:200-1:1000 |
Background
Key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.