P53 Polyclonal Antibody Storage Store at -20°C
SKU: E-AB-32469-200
P53 Polyclonal Antibody Storage Store at -20°C
| SKU # | E-AB-32469 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Applications | WB, IHC-p, IF |
Product Details
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC-p, IF |
| Clonality | Polyclonal |
| Immunogen | Synthesized peptide derived from the C-terminal region of human p53 |
| Abbre | P53 |
| Synonyms | Antigen NY-CO-13, BCC7, Cellular tumor antigen p53, FLJ92943, LFS1, Mutant tumor protein 53, P53, Phosphoprotein p53, TRP53, Tp53, Transformation related protein 53, Tumor protein 53, Tumor protein p53, Tumor suppressor p53, p53, p53 tumor suppressor |
| Swissprot | |
| Calculated MW | 44 kDa |
| Observed MW | 43 kDa |
| Cellular Localization | Cytoplasm, Cytoplasm, Nucleus, Nucleus>PML body, Endoplasmic reticulum, Interaction with BANP promotes nuclear localization, Recruited into PML bodies together with CHEK2, Nucleus, Cytoplasm, Localized in both nucleus and cytoplasm in most cells, In some cells, forms foci in the nucleus that are different from nucleoli, Nucleus, Cytoplasm, Localized in the nucleus in most cells but found in the cytoplasm in some cells, Nucleus, Cytoplasm, Localized mainly in the nucleus with minor staining in the cytoplasm, Nucleus, Cytoplasm, Predominantly nuclear but localizes to the cytoplasm when expressed with isoform 4 and Nucleus, Cytoplasm, Predominantly nuclear but translocates to the cytoplasm following cell stress. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Cell Biology, Epigenetics and Nuclear Signaling |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
Related Reagents
| Applications | Recommended Dilution |
| WB | 1:500-1:2000 |
| IHC | 1:100-1:300 |
| IF | 1:200-1:1000 |
Background
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.