Oxalate (Oxalic Acid) Colorimetric Assay Kit

$ 1,105⁹⁵

Oxalate (Oxalic Acid) Colorimetric Assay Kit

SKU #	E-BC-K892-M
Detection Instrument	Micropipettor, Microplate reader (545-555 nm, optimum wavelength: 550 nm)
Detection method	Colorimetric method

Product Details

Properties

Sample type	Animal urine, serum, plasma, plant tissue
Sensitivity	0.02 mmol/L
Detection range	0.02-1 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	35 min
Precision	Average inter-assay CV: 4.700% \| Average intra-assay CV: 3%
Other instruments required	Micropipettor, Centrifuge, 37℃ incubator
Storage	-20℃
Valid period	12 months

Dilution of Sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range ( 0.02-1 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human urine	1-2
Human plasma	1
10% Epipremnum aureum tissue homogenate	2-3
Rats plasma	1

Note: The diluent is double distilled water.

Detection Principle

Oxalate oxidase catalyzes the oxidation of oxalate to produce hydrogen peroxide and carbon dioxide. Under the action of POD, hydrogen peroxide reacts with chromogenic substances to produce colored products. There is a specific absorption peak at 550 nm, and the color depth is proportional to the content of oxalate.

Kit Components & Storage

Item	Component	Size (96 T)	Storage
Reagent 1	Working Solution	12 mL × 1 vial	-20℃, 12 months
Reagent 2	Chromogenic Agent A	12 mL × 1 vial	-20℃, 12 months, shading light
Reagent 3	Chromogenic Agent B	12 mL × 1 vial	-20℃, 12 months, shading light
Reagent 4	Enzyme Reagent A	Powder × 2 vials	-20℃, 12 months
Reagent 5	Enzyme Reagent B	Powder × 2 vials	-20℃, 12 months
Reagent 6	Regulator	12 mL × 1 vial	-20℃, 12 months
Reagent 7	Clarificant	2 mL × 1 vial	-20℃, 12 months
Reagent 8	1 mmol/L Standard	2 mL × 1 vial	-20℃, 12 months
	Microplate	96 wells	No requirement
	Plate Sealer	2 pieces

Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.

Technical Data:

Parameter:

Intra-assay Precision

Three human serum samples were assayed in replicates of 20 to determine precision within an assay (CV = Coefficient of Variation).

Parameters	Sample 1	Sample 2	Sample 3
Mean (mmol/L)	0.08	0.54	0.88
%CV	3.5	3.1	2.4

Inter-assay Precision

Three human serum samples were assayed 20 times in duplicate by three operators to determine precision between assays.

Parameters	Sample 1	Sample 2	Sample 3
Mean (mmol/L)	0.08	0.54	0.88
%CV	4.8	5.2	4.1

Recovery

Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 95%.

	Standard 1	Standard 2	Standard 3
Expected Conc. (mmol/L)	0.35	0.55	0.75
Observed Conc. (mmol/L)	0.3	0.5	0.7
Recovery rate(%)	96	94	95

Sensitivity

The analytical sensitivity of the assay is 0.02 mmol/L. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.

Standard Curve

As the OD value of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique or temperature effects), so the standard curve and data are provided as below for reference only:

Concentration (mmol/L)	0	0.2	0.4	0.5	0.6	0.7	0.8	1.0
Average OD	0.046	0.137	0.228	0.268	0.316	0.353	0.390	0.468
Absoluted OD	0.000	0.090	0.182	0.222	0.270	0.307	0.344	0.422