NFκB-p105/p50 Polyclonal Antibody
SKU: E-AB-32226-200
NFκB-p105/p50 Polyclonal Antibody
| SKU # | E-AB-32226 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Applications | WB, IHC-p, IF |
Product Details
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB, IHC-p, IF |
| Clonality | Polyclonal |
| Immunogen | Synthesized peptide derived from human NFκB-p105/p50 around the non-phosphorylation site of Ser337. |
| Abbre | NFκB-p105/p50 |
| Synonyms | DKFZp686C01211, DNA binding factor KBF1, DNA binding factor KBF1 EBP1, DNA-binding factor KBF1, EBP 1, EBP-1, EBP1, KBF1, MGC54151, NF kB1, NF kappa B, NF kappaB, NF kappabeta, NFKB 1, NFKB p105, NFKB p50, NFKB1, NFkappaB, Nfkb1, Nuclear factor kappa B DNA binding subu, nf b |
| Swissprot | |
| Calculated MW | 105 kDa |
| Observed MW | 50 ,110kDa |
| Cellular Localization | Nucleus. Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Cell Biology, Epigenetics and Nuclear Signaling, Immunology, Signal Transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
Related Reagents
| Applications | Recommended Dilution |
| WB | 1:500-1:2000 |
| IHC | 1:100-1:300 |
| IF | 1:200-1:1000 |
Background
NFkB is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. Activated NFkB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFkB has been associated with a number of inflammatory diseases while persistent inhibition of NFkB leads to inappropriate immune cell development or delayed cell growth. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. This antibody can bind both p105 and p50 isoforms of NFKB1.