MSE PRO Zebrafish GH(Growth Hormone) ELISA Kit
SKU: EK0064668-96T
MSE PRO™ Zebrafish GH(Growth Hormone) ELISA Kit
Specifications:
| Product name | Zebrafish GH(Growth Hormone) ELISA Kit |
| Reactivity | Zebrafish |
| Alternative Names | GH1; GH-N; GHN; hGH-N; Somatotropin; Hygetropin; Jintropin; Kigtropin; Pituitary Growth Hormone; Growth Hormone, Normal |
| Assay Type | Sandwich |
| Sensitivity | 57.3 pg/mL |
| Standard | 10 ng/mL |
| Detection Range | 0.16-10 ng/mL |
| Sample Type | serum, plasma and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Endocrinology; Neuro science; Hormone metabolism |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Zebrafish GH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish GH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Zebrafish GH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish GH in the samples is then determined by comparing the OD of the samples to the standard curve. |