MSE PRO VMA(Vanillylmandelic Acid) ELISA Kit
SKU: EK0064270-48T
MSE PRO™ VMA (Vanillylmandelic Acid) ELISA Kit
Specifications:
| Product name | VMA (Vanillylmandelic Acid) ELISA Kit |
| Reactivity | General |
| Alternative Names | Vanillyl Mandelic Acid |
| Assay Type | Competitive Inhibition |
| Sensitivity | 43 ng/mL |
| Standard | 10000 ng/mL |
| Detection Range | 156.25-10000 ng/mL |
| Sample type | urine and biological agents |
| Assay length | 2h |
| Research Area | Metabolic pathway; Endocrinology; Cardiovascular biology; Hepatology; Gastroenterology; Hormone metabolism |
| Test principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with VMA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to VMA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VMA in the samples is then determined by comparing the OD of the samples to the standard curve. |