MSE PRO Sheep GC(Glucagon) ELISA Kit
SKU: EK0064618-96T
MSE PRO™ Sheep GC(Glucagon) ELISA Kit
Specifications:
| Product name | Sheep GC(Glucagon) ELISA Kit |
| Reactivity | Sheep |
| Alternative Names | GCG; GLP1; GLP2; GRPP; Glicentin-Related Polypeptide; Glucagen; Oxyntomodulin; Incretin hormone |
| Assay Type | Competitive Inhibition |
| Sensitivity | 12.7 pg/mL |
| Standard | 2000 pg/mL |
| Detection Range | 31.25-2000 pg/mL |
| Sample Type | serum, plasma and other biological fluids |
| Assay Length | 2h |
| Research Area | Endocrinology |
| Test principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Sheep GC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep GC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep GC in the samples is then determined by comparing the OD of the samples to the standard curve. |