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MSE PRO Sheep C3a(Complement Component 3a) ELISA Kit

MSE PRO Sheep C3a(Complement Component 3a) ELISA Kit

SKU: EK0064890-96T

  • $ 45795

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MSE PRO™ Sheep C3a(Complement Component 3a) ELISA Kit

Specifications:

Product name Sheep C3a(Complement Component 3a) ELISA Kit
Reactivity Sheep
Alternative Names Complement Component 3a
Assay Type Sandwich
Sensitivity 3.73 ng/mL
Standard 600 ng/mL
Detection Range 9.38-600 ng/mL
Sample Type serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length 3.5h
Research Area Infection immunity; Immune molecule; Autoimmunity
Test principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep C3a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep C3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep C3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep C3a in the samples is then determined by comparing the OD of the samples to the standard curve.