MSE PRO Rat SREBP-1C(Sterol Regulatory Element-Binding Protein 1C) ELISA Kit
SKU: EK0064579-96T
MSE PRO™ Rat SREBP-1C(Sterol Regulatory Element-Binding Protein 1C) ELISA Kit
Specifications:
| Product name | Rat SREBP-1C(Sterol Regulatory Element-Binding Protein 1C) ELISA Kit |
| Reactivity | Rat |
| Alternative Names | ADD-1; ADD1; SREBP-1; SREBP-1c; Srebp1 |
| Assay Type | Sandwich |
| Sensitivity | 18.3 pg/mL |
| Standard | 4000 pg/mL |
| Detection Range | 62.5-4000 pg/mL |
| Sample Type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Cardiovascular; Epigenetics and Nuclear Signaling; Cancer; Metabolism |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SREBP-1C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SREBP-1C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SREBP-1C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SREBP-1C in the samples is then determined by comparing the OD of the samples to the standard curve. |