MSE PRO Rat Smad3(Mothers Against Decapentaplegic Homolog 3) ELISA Kit
Regular price
$ 264.95
Couldn't load pickup availability
Still have questions? Ask our experts!
MSE PRO™ Rat Smad3(Mothers Against Decapentaplegic Homolog 3) ELISA Kit
Specifications:
| Product name | Rat Smad3(Mothers Against Decapentaplegic Homolog 3) ELISA Kit |
| Reactivity | Rat |
| Alternative Names | JV15-2; MADH3 |
| Assay Type | Sandwich |
| Sensitivity | 0.079 ng/mL |
| Standard | 10 ng/mL |
| Detection Range | 0.16-10 ng/mL |
| Sample Type | tissue homogenates, cell lysates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Signal transduction; Tumor immunity |
| Uniprot ID | P84025 |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Smad3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Smad3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Smad3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Smad3 in the samples is then determined by comparing the OD of the samples to the standard curve. |